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- EMDB-32791: Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Dis-E -
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Open data
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Basic information
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Title | Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Dis-E | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
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Method | ![]() ![]() | |||||||||
![]() | Cheng J / La Venuta G / Lau B / Berninghausen O / Beckmann R / Hurt E | |||||||||
Funding support | 1 items
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![]() | ![]() Title: In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation. Authors: Jingdong Cheng / Giuseppe La Venuta / Benjamin Lau / Otto Berninghausen / Roland Beckmann / Ed Hurt / ![]() ![]() Abstract: The transition of the 90S to the pre-40S pre-ribosome is a decisive step in eukaryotic small subunit biogenesis leading to a first pre-40S intermediate (state Dis-C or primordial pre-40S), where the ...The transition of the 90S to the pre-40S pre-ribosome is a decisive step in eukaryotic small subunit biogenesis leading to a first pre-40S intermediate (state Dis-C or primordial pre-40S), where the U3 snoRNA keeps the nascent 18S rRNA locally immature. We in vitro reconstitute the ATP-dependent U3 release from this particle, catalyzed by the helicase Dhr1, and follow this process by cryo-EM revealing two successive pre-40S intermediates, Dis-D and Dis-E. The latter has lost not only U3 but all residual 90S factors including the GTPase Bms1. In vitro remodeling likewise induced the formation of the central pseudoknot, a universally conserved tertiary RNA structure that comprises the core of the small subunit decoding center. Thus, we could structurally reveal a key tertiary RNA folding step that is essential to form the active 40S subunit. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 105 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 26.8 KB 26.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.8 KB | Display | ![]() |
Images | ![]() | 87.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7wtmMC ![]() 7wtlC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.047 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
+Entire : Yeast pre-40S ribosomal subunit
+Supramolecule #1: Yeast pre-40S ribosomal subunit
+Macromolecule #1: 18S rRNA
+Macromolecule #2: 40S ribosomal protein S1-A
+Macromolecule #3: 40S ribosomal protein S4-A
+Macromolecule #4: 40S ribosomal protein S6-A
+Macromolecule #5: 40S ribosomal protein S7-A
+Macromolecule #6: 40S ribosomal protein S8-A
+Macromolecule #7: 40S ribosomal protein S9-A
+Macromolecule #8: 40S ribosomal protein S11-A
+Macromolecule #9: 40S ribosomal protein S13
+Macromolecule #10: 40S ribosomal protein S14-A
+Macromolecule #11: 40S ribosomal protein S22-A
+Macromolecule #12: 40S ribosomal protein S23-A
+Macromolecule #13: 40S ribosomal protein S24-A
+Macromolecule #14: 40S ribosomal protein S27-A
+Macromolecule #15: 40S ribosomal protein S30-A
+Macromolecule #16: Pre-rRNA-processing protein PNO1
+Macromolecule #17: Dimethyladenosine transferase
+Macromolecule #18: ZINC ION
-Experimental details
-Structure determination
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 44.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |