PDB-2qzf: SCR1 of DAF from 1ojv fitted into cryoEM density

Entry
Summary
Database / ID	PORTEIN DATA BANK (PDB) / 2qzf
Title	SCR1 of DAF from 1ojv fitted into cryoEM density
Descriptor	Complement decay-accelerating factor
Keywords	Immune system, SCR1 of DAF from structure 1ojv fitted into cryoEM density, Blood group antigen, Complement pathway, Glycoprotein, GPI-anchor, Immune response, Innate immunity, Lipoprotein, Membrane, Sushi
PDBj Mine pages	Summary, Structural Details, Experimental Details, Functional Details
Other databases	RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Structure Visualization
Movies	Movie Page #1: Depositted structure unit, Made by Jmol #2: Superimposing with simplified surface model of EM map, EMDB-1412, Made by Jmol #3: With PDB-2qzd, PDB-2qzh, Made by Jmol #4: Superimposing with EM 3D map: EMDB-1412 (with PDB-2qzd, PDB-2qzh), Made by UCSF CHIMERA
Structure viewers	Yorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries	EMDB-1411 Fit EMDB-1412 Fit Fit: target map of fitting
Similar strucutres (beta)	Diagram PDB-2r1c PDB-2qzd List of similar structure data about Omokage system

Article
Citation - primary
Article	J.Virol., Vol. 81, Page: 12927 - 12935, Year: 2007,
Title	Interaction of decay-accelerating factor with coxsackievirus b3.
Authors	Hafenstein, S., Bowman, V.D., Chipman, P.R., Kelly, C.M., Lin, F., Medof, M.E., Rossmann, M.G.
Links	DOI: 10.1128/JVI.00931-07

Components
ID 1 : CD55 antigen
Image
Description	Complement decay-accelerating factor
Type	polypeptide(L)
Fragment	SCR1 domain
Formula weight	6884.752 Da
Number of molecules	1
ID	1
Source	Method: Isolated from a genetically manipulated source Gene: human, Homo, ID:9606, Homo sapiens Host: Escherichia, ID:562, Escherichia coli
Links	UniProt: P08174, Sequence view

Sample
Assembly
Aggregation state	PARTICLE
Details	one DAF binds each binding site, one per each protomer
Name	coxsackievirus B3, RD strain, complexed with decay-accelerating factor
Entity assembly
Assembly-ID	1
GO-ID	GO:0005887
InterPro-ID	IPR:000436
Name	Complement decay-accelerating factor
Buffer
Name	50mM MES
Experiment
Reconstruction method	SINGLE PARTICLE
Specimen type	VITREOUS ICE (CRYO EM)
Sample preparation
pH	6.0
Sample concentration	2 mg/ml
Sample support
Details	quantifoils
Vitrification
Details	blot before plunging
Experiment
Method	ELECTRON MICROSCOPY