National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)
United States
National Institutes of Health/National Institute of Mental Health (NIH/NIMH)
Citation
Journal: Nat Struct Mol Biol / Year: 2020 Title: Structure and assembly of calcium homeostasis modulator proteins. Authors: Johanna L Syrjanen / Kevin Michalski / Tsung-Han Chou / Timothy Grant / Shanlin Rao / Noriko Simorowski / Stephen J Tucker / Nikolaus Grigorieff / Hiro Furukawa / Abstract: The biological membranes of many cell types contain large-pore channels through which a wide variety of ions and metabolites permeate. Examples include connexin, innexin and pannexin, which form gap ...The biological membranes of many cell types contain large-pore channels through which a wide variety of ions and metabolites permeate. Examples include connexin, innexin and pannexin, which form gap junctions and/or bona fide cell surface channels. The most recently identified large-pore channels are the calcium homeostasis modulators (CALHMs), through which ions and ATP permeate in a voltage-dependent manner to control neuronal excitability, taste signaling and pathologies of depression and Alzheimer's disease. Despite such critical biological roles, the structures and patterns of their oligomeric assembly remain unclear. Here, we reveal the structures of two CALHMs, chicken CALHM1 and human CALHM2, by single-particle cryo-electron microscopy (cryo-EM), which show novel assembly of the four transmembrane helices into channels of octamers and undecamers, respectively. Furthermore, molecular dynamics simulations suggest that lipids can favorably assemble into a bilayer within the larger CALHM2 pore, but not within CALHM1, demonstrating the potential correlation between pore size, lipid accommodation and channel activity.
History
Deposition
Dec 17, 2019
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Header (metadata) release
Jan 15, 2020
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Map release
Jan 29, 2020
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Update
Nov 25, 2020
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Current status
Nov 25, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Entire : Undecameric assembly of human calcium homeostasis modulator prote...
Entire
Name: Undecameric assembly of human calcium homeostasis modulator protein 2 (CALHM2) in EDTA
Components
Complex: Undecameric assembly of human calcium homeostasis modulator protein 2 (CALHM2) in EDTA
Protein or peptide: Calcium homeostasis modulator protein 2
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Supramolecule #1: Undecameric assembly of human calcium homeostasis modulator prote...
Supramolecule
Name: Undecameric assembly of human calcium homeostasis modulator protein 2 (CALHM2) in EDTA type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)
Organism: Homo sapiens (human)
Recombinant expression
Organism: Spodoptera frugiperda (fall armyworm)
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Macromolecule #1: Calcium homeostasis modulator protein 2
Macromolecule
Name: Calcium homeostasis modulator protein 2 / type: protein_or_peptide / ID: 1 / Number of copies: 11 / Enantiomer: LEVO
Cryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 288.15 K / Instrument: FEI VITROBOT MARK IV / Details: Blot for 4 sec before plunging.
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Electron microscopy
Microscope
FEI TITAN KRIOS
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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