Journal: Proc Natl Acad Sci U S A / Year: 2020 Title: Structure of a rabies virus polymerase complex from electron cryo-microscopy. Authors: Joshua A Horwitz / Simon Jenni / Stephen C Harrison / Sean P J Whelan / Abstract: Nonsegmented negative-stranded (NNS) RNA viruses, among them the virus that causes rabies (RABV), include many deadly human pathogens. The large polymerase (L) proteins of NNS RNA viruses carry all ...Nonsegmented negative-stranded (NNS) RNA viruses, among them the virus that causes rabies (RABV), include many deadly human pathogens. The large polymerase (L) proteins of NNS RNA viruses carry all of the enzymatic functions required for viral messenger RNA (mRNA) transcription and replication: RNA polymerization, mRNA capping, and cap methylation. We describe here a complete structure of RABV L bound with its phosphoprotein cofactor (P), determined by electron cryo-microscopy at 3.3 Å resolution. The complex closely resembles the vesicular stomatitis virus (VSV) L-P, the one other known full-length NNS-RNA L-protein structure, with key local differences (e.g., in L-P interactions). Like the VSV L-P structure, the RABV complex analyzed here represents a preinitiation conformation. Comparison with the likely elongation state, seen in two structures of pneumovirus L-P complexes, suggests differences between priming/initiation and elongation complexes. Analysis of internal cavities within RABV L suggests distinct template and product entry and exit pathways during transcription and replication.
History
Deposition
Sep 20, 2019
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Header (metadata) release
Oct 2, 2019
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Map release
Feb 5, 2020
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Update
Mar 20, 2024
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Current status
Mar 20, 2024
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 72.0 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
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Image processing
Startup model
Type of model: INSILICO MODEL
Initial angle assignment
Type: RANDOM ASSIGNMENT
Final angle assignment
Type: MAXIMUM LIKELIHOOD
Final reconstruction
Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF Details: cisTEM was used for the final reconstruction and FSC curve/resolution analysis. Number images used: 44500
FSC plot (resolution estimation)
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