+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1c2x | ||||||
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タイトル | 5S RRNA STRUCTURE FITTED TO A CRYO-ELECTRON MICROSCOPIC MAP AT 7.5 ANGSTROMS RESOLUTION | ||||||
要素 | 5S RIBOSOMAL RNA | ||||||
キーワード | RIBOSOME / 5S RRNA / 23S RRNA / LARGE RIBOSOMAL SUBUNIT / PROTEIN BIOSYNTHESIS / RIBONUCLEIC ACID / EM-RECONSTRUCTION / ATOMIC STRUCTURE / 3D ARRANGEMENT / FITTING | ||||||
機能・相同性 | RNA / RNA (> 10) / RNA (> 100) 機能・相同性情報 | ||||||
生物種 | Escherichia coli (大腸菌) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.5 Å | ||||||
データ登録者 | Brimacombe, R. / Mueller, F. | ||||||
引用 | ジャーナル: J Mol Biol / 年: 2000 タイトル: The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution. 著者: F Mueller / I Sommer / P Baranov / R Matadeen / M Stoldt / J Wöhnert / M Görlach / M van Heel / R Brimacombe / 要旨: The Escherichia coli 23 S and 5 S rRNA molecules have been fitted helix by helix to a cryo-electron microscopic (EM) reconstruction of the 50 S ribosomal subunit, using an unfiltered version of the ...The Escherichia coli 23 S and 5 S rRNA molecules have been fitted helix by helix to a cryo-electron microscopic (EM) reconstruction of the 50 S ribosomal subunit, using an unfiltered version of the recently published 50 S reconstruction at 7.5 A resolution. At this resolution, the EM density shows a well-defined network of fine structural elements, in which the major and minor grooves of the rRNA helices can be discerned at many locations. The 3D folding of the rRNA molecules within this EM density is constrained by their well-established secondary structures, and further constraints are provided by intra and inter-rRNA crosslinking data, as well as by tertiary interactions and pseudoknots. RNA-protein cross-link and foot-print sites on the 23 S and 5 S rRNA were used to position the rRNA elements concerned in relation to the known arrangement of the ribosomal proteins as determined by immuno-electron microscopy. The published X-ray or NMR structures of seven 50 S ribosomal proteins or RNA-protein complexes were incorporated into the EM density. The 3D locations of cross-link and foot-print sites to the 23 S rRNA from tRNA bound to the ribosomal A, P or E sites were correlated with the positions of the tRNA molecules directly observed in earlier reconstructions of the 70 S ribosome at 13 A or 20 A. Similarly, the positions of cross-link sites within the peptidyl transferase ring of the 23 S rRNA from the aminoacyl residue of tRNA were correlated with the locations of the CCA ends of the A and P site tRNA. Sites on the 23 S rRNA that are cross-linked to the N termini of peptides of different lengths were all found to lie within or close to the internal tunnel connecting the peptidyl transferase region with the presumed peptide exit site on the solvent side of the 50 S subunit. The post-transcriptionally modified bases in the 23 S rRNA form a cluster close to the peptidyl transferase area. The minimum conserved core elements of the secondary structure of the 23 S rRNA form a compact block within the 3D structure and, conversely, the points corresponding to the locations of expansion segments in 28 S rRNA all lie on the outside of the structure. #1: ジャーナル: J.Mol.Biol. / 年: 1997 タイトル: A New Model for the Three-Dimensional Folding of Escherichia Coli 16S Ribosomal RNA. I. Fitting the RNA to a 3D Electron Microscopic Map at 20 Angstroms 著者: Mueller, F. / Brimacombe, R. #2: ジャーナル: J.Mol.Biol. / 年: 1997 タイトル: A New Model for the Three-Dimensional Folding of Escherichia Coli 16S Ribosomal RNA. II. The RNA-Protein Interaction Data 著者: Mueller, F. / Brimacombe, R. #3: ジャーナル: J.Mol.Biol. / 年: 1997 タイトル: A New Model for the Three-Dimensional Folding of Escherichia Coli 16S Ribosomal RNA. III. The Topography of the Functional Centre 著者: Mueller, F. / Stark, H. / Van Heel, M. / Rinke-Appel, J. / Brimacombe, R. #4: ジャーナル: Structure / 年: 1999 タイトル: The Escherichia coli large ribosomal subunit at 7.5 A resolution 著者: Matadeen, R. / Patwardhan, A. / Gowen, B. / Orlova, E.V. / Pape, T. / Cuff, M. / Mueller, F. / Brimacombe, R. / van Heel, M. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1c2x.cif.gz | 69.7 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1c2x.ent.gz | 45.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1c2x.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1c2x_validation.pdf.gz | 334.4 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1c2x_full_validation.pdf.gz | 410.6 KB | 表示 | |
XML形式データ | 1c2x_validation.xml.gz | 12.1 KB | 表示 | |
CIF形式データ | 1c2x_validation.cif.gz | 17 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/c2/1c2x ftp://data.pdbj.org/pub/pdb/validation_reports/c2/1c2x | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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-要素
#1: RNA鎖 | 分子量: 38790.090 Da / 分子数: 1 / 由来タイプ: 天然 / 詳細: THIRD OF THE 3 RRNA CHAINS OF THE RIBOSOME / 由来: (天然) Escherichia coli (大腸菌) / 細胞内の位置: CYTOPLASM |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: 50S Ribosomal Subunit / タイプ: RIBOSOME |
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試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
結晶 | 解説: THE CRYST1 AND SCALE RECORDS ARE MEANINGLESS. |
-電子顕微鏡撮影
顕微鏡 | モデル: FEI/PHILIPS CM200FEG / 詳細: from Structure 1999 citation |
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電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 200 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD / 倍率(公称値): 38000 X / 最大 デフォーカス(公称値): 2000 nm / 最小 デフォーカス(公称値): 1000 nm |
試料ホルダ | 試料ホルダーモデル: GATAN LIQUID NITROGEN |
撮影 | フィルム・検出器のモデル: GENERIC FILM |
画像スキャン | Scanner model: IMAGE SCIENCE PATCHWORK DENSITOMETER |
-解析
ソフトウェア | 名称: ERNA-3D / 分類: 精密化 | |||||||||||||||||||||
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EMソフトウェア | 名称: IMAGIC / バージョン: 5 / カテゴリ: CTF補正 | |||||||||||||||||||||
粒子像の選択 | 選択した粒子像数: 16000 / 詳細: from seven micrographs | |||||||||||||||||||||
対称性 | 点対称性: C1 (非対称) | |||||||||||||||||||||
3次元再構成 | 解像度: 7.5 Å / 解像度の算出法: FSC 3 SIGMA CUT-OFF / 粒子像の数: 16000 / 対称性のタイプ: POINT | |||||||||||||||||||||
原子モデル構築 | 空間: REAL 詳細: DETAILS--THIS FILE HAS BEEN GENERATED BY THE USE OF ALL RELEVANT BIOCHEMICAL CONSTRAINTS AND THE CONSTRAINTS GIVEN BY THE ELECTRON DENSITY CONTOUR OF THE RIBOSOME, WHICH WAS DERIVED FROM THE ...詳細: DETAILS--THIS FILE HAS BEEN GENERATED BY THE USE OF ALL RELEVANT BIOCHEMICAL CONSTRAINTS AND THE CONSTRAINTS GIVEN BY THE ELECTRON DENSITY CONTOUR OF THE RIBOSOME, WHICH WAS DERIVED FROM THE CRYO-ELECTRON MICROSCOPIC RECONSTRUCTION. THIS FILE IS PART OF A SET OF ALL THREE RIBONUCLEIC ACID CHAINS OF THE RIBOSOME TOGETHER WITH A NUMBER OF RIBOSOMAL PROTEINS WHICH ARE ALSO DEPOSITED WITH THE PDB DATA BANK. THE ATOMIC COORDINATES OF ALL THESE RIBOSOMAL COMPONENTS REFLECT THEIR POSITIONS IN THE 70S RIBOSOME. | |||||||||||||||||||||
原子モデル構築 |
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精密化 | 最高解像度: 7.5 Å | |||||||||||||||||||||
精密化ステップ | サイクル: LAST / 最高解像度: 7.5 Å
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