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Yorodumi- EMDB-1919: Visualizing branched actin filaments in lamellipodia by electron ... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-1919 | |||||||||
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Title | Visualizing branched actin filaments in lamellipodia by electron tomography | |||||||||
Map data | Tomogram of a lamellipodium of a 3T3 cell spread on polylysine | |||||||||
Sample |
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Keywords | actin / branching / Arp2/3 / lamellipodium | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | electron tomography / negative staining | |||||||||
Authors | Small JV / Winkler C / Vinzenz M / Schmeiser C | |||||||||
Citation | Journal: Nat Cell Biol / Year: 2010 Title: Electron tomography reveals unbranched networks of actin filaments in lamellipodia. Authors: Edit Urban / Sonja Jacob / Maria Nemethova / Guenter P Resch / J Victor Small / Abstract: Eukaryotic cells can initiate movement using the forces exerted by polymerizing actin filaments to extend lamellipodial and filopodial protrusions. In the current model, actin filaments in ...Eukaryotic cells can initiate movement using the forces exerted by polymerizing actin filaments to extend lamellipodial and filopodial protrusions. In the current model, actin filaments in lamellipodia are organized in a branched, dendritic network. We applied electron tomography to vitreously frozen 'live' cells, fixed cells and cytoskeletons, embedded in vitreous ice or in deep-negative stain. In lamellipodia from four cell types, including rapidly migrating fish keratocytes, we found that actin filaments are almost exclusively unbranched. The vast majority of apparent filament junctions proved to be overlapping filaments, rather than branched end-to-side junctions. Analysis of the tomograms revealed that actin filaments terminate at the membrane interface within a zone several hundred nanometres wide at the lamellipodium front, and yielded the first direct measurements of filament densities. Actin filament pairs were also identified as lamellipodium components and bundle precursors. These data provide a new structural basis for understanding actin-driven protrusion during cell migration. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_1919.map.gz | 154.3 MB | EMDB map data format | |
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Header (meta data) | emd-1919-v30.xml emd-1919.xml | 7.7 KB 7.7 KB | Display Display | EMDB header |
Images | EMD-1919.tif | 745.5 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-1919 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1919 | HTTPS FTP |
-Validation report
Summary document | emd_1919_validation.pdf.gz | 155.3 KB | Display | EMDB validaton report |
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Full document | emd_1919_full_validation.pdf.gz | 154.4 KB | Display | |
Data in XML | emd_1919_validation.xml.gz | 5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1919 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1919 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_1919.map.gz / Format: CCP4 / Size: 252.3 MB / Type: IMAGE STORED AS SIGNED BYTE | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Tomogram of a lamellipodium of a 3T3 cell spread on polylysine | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 7.46 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Tomogram of a lamellipodium of a 3T3 cell
Entire | Name: Tomogram of a lamellipodium of a 3T3 cell |
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Components |
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-Supramolecule #1000: Tomogram of a lamellipodium of a 3T3 cell
Supramolecule | Name: Tomogram of a lamellipodium of a 3T3 cell / type: sample / ID: 1000 / Number unique components: 1 |
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-Supramolecule #1: Actin
Supramolecule | Name: Actin / type: organelle_or_cellular_component / ID: 1 / Name.synonym: Actin / Oligomeric state: Multimer / Recombinant expression: No / Database: NCBI |
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Source (natural) | Organism: Mus musculus (house mouse) / synonym: House mouse / Cell: NIH 3t3 / Location in cell: Lamellipodium |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | electron tomography |
-Sample preparation
Staining | Type: NEGATIVE / Details: 4% SST |
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Vitrification | Cryogen name: NONE / Instrument: OTHER |
-Electron microscopy
Microscope | FEI POLARA 300 |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal magnification: 31000 |
Sample stage | Specimen holder: Eucentric / Specimen holder model: PHILIPS ROTATION HOLDER / Tilt series - Axis1 - Min angle: -60 ° / Tilt series - Axis1 - Max angle: 60 ° |
Experimental equipment | Model: Tecnai Polara / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Software - Name: IMOD / Details: double tilt |
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