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- EMDB-1814: Structural Comparison of HIV-1 Envelope Spikes with and without t... -

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Basic information

Entry
Database: EMDB / ID: EMD-1814
TitleStructural Comparison of HIV-1 Envelope Spikes with and without the V1V2 Loop
Map dataThis is a map for HIV-1 Envelope spike with the v1v2 loop deleted
Sample
  • Sample: HIV-1 R3A deleteV1V2 virus
  • Organelle or cellular component: Envelope glycoprotein
KeywordsStructural classification
Biological speciesHuman immunodeficiency virus 1
Methodsubtomogram averaging / cryo EM / Resolution: 33.0 Å
AuthorsHu G / Liu J / Taylor KA / Roux KH
CitationJournal: J Virol / Year: 2011
Title: Structural comparison of HIV-1 envelope spikes with and without the V1/V2 loop.
Authors: Guiqing Hu / Jun Liu / Kenneth A Taylor / Kenneth H Roux /
Abstract: We have used cryoelectron tomography of vitreous-ice-embedded HIV-1 virions to compare the envelope (Env) spikes of a wild-type strain with those of a mutant strain in which the V1/V2 loop has been ...We have used cryoelectron tomography of vitreous-ice-embedded HIV-1 virions to compare the envelope (Env) spikes of a wild-type strain with those of a mutant strain in which the V1/V2 loop has been deleted. Deletion of V1/V2 results in a spike with far more structural heterogeneity than is observed in the wild type, likely reflecting greatly enhanced gp120 protomer flexibility. A major difference between the two forms is a pronounced loss of mass from the "peak" of the native Env spike. The apparent loss of contact among three gp120 protomers likely accounts for the more open structure, heterogeneity in configuration, and previous observations that broadly neutralizing epitopes and reactive sites on other structural elements are more exposed in such constructs.
History
DepositionOct 25, 2010-
Header (metadata) releaseNov 1, 2010-
Map releaseFeb 17, 2012-
UpdateMar 14, 2012-
Current statusMar 14, 2012Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.25
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.25
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

image.png

Downloads & links

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Map

FileDownload / File: emd_1814.map.gz / Format: CCP4 / Size: 230.5 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThis is a map for HIV-1 Envelope spike with the v1v2 loop deleted
Voxel sizeX=Y=Z: 4.6 Å
Density
Contour LevelBy AUTHOR: 0.25 / Movie #1: 0.25
Minimum - Maximum-1.44338107 - 1.79818022
Average (Standard dev.)-0.03480374 (±0.49674973)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions353549
Spacing353549
CellA: 161.0 Å / B: 161.0 Å / C: 225.4 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.64.64.6
M x/y/z353549
origin x/y/z0.0000.0000.000
length x/y/z161.000161.000225.400
α/β/γ90.00090.00090.000
start NX/NY/NZ-184-184-183
NX/NY/NZ368368368
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS353549
D min/max/mean-1.4431.798-0.035

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Supplemental data

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Sample components

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Entire : HIV-1 R3A deleteV1V2 virus

EntireName: HIV-1 R3A deleteV1V2 virus
Components
  • Sample: HIV-1 R3A deleteV1V2 virus
  • Organelle or cellular component: Envelope glycoprotein

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Supramolecule #1000: HIV-1 R3A deleteV1V2 virus

SupramoleculeName: HIV-1 R3A deleteV1V2 virus / type: sample / ID: 1000 / Details: Sample is ice-embeded / Number unique components: 1

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Supramolecule #1: Envelope glycoprotein

SupramoleculeName: Envelope glycoprotein / type: organelle_or_cellular_component / ID: 1 / Name.synonym: HIV-1 virus / Recombinant expression: Yes / Database: NCBI
Source (natural)Organism: Human immunodeficiency virus 1 / Strain: R3A

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging

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Sample preparation

VitrificationCryogen name: ETHANE / Instrument: OTHER

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Sample stageSpecimen holder: Multi-specimen holer / Specimen holder model: OTHER
Image recordingCategory: CCD / Film or detector model: GENERIC CCD / Average electron dose: 100 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

Final reconstructionAlgorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 33.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: Protomo and I3

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Atomic model buiding 1

Initial modelPDB ID:

3fus

RefinementSpace: REAL

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