+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-13198 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of human TTYH3 in Ca2+ and GDN | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
Function / homology | ![]() volume-sensitive chloride channel activity / intracellularly calcium-gated chloride channel activity / chloride transport / ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Sukalskaia A / Straub MS / Sawicka M / Deneka D / Dutzler R | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Cryo-EM structures of the TTYH family reveal a novel architecture for lipid interactions. Authors: Anastasiia Sukalskaia / Monique S Straub / Dawid Deneka / Marta Sawicka / Raimund Dutzler / ![]() Abstract: The Tweety homologs (TTYHs) are members of a conserved family of eukaryotic membrane proteins that are abundant in the brain. The three human paralogs were assigned to function as anion channels that ...The Tweety homologs (TTYHs) are members of a conserved family of eukaryotic membrane proteins that are abundant in the brain. The three human paralogs were assigned to function as anion channels that are either activated by Ca or cell swelling. To uncover their unknown architecture and its relationship to function, we have determined the structures of human TTYH1-3 by cryo-electron microscopy. All structures display equivalent features of a dimeric membrane protein that contains five transmembrane segments and an extended extracellular domain. As none of the proteins shows attributes reminiscent of an anion channel, we revisited functional experiments and did not find any indication of ion conduction. Instead, we find density in an extended hydrophobic pocket contained in the extracellular domain that emerges from the lipid bilayer, which suggests a role of TTYH proteins in the interaction with lipid-like compounds residing in the membrane. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 38 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 15.2 KB 15.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.1 KB | Display | ![]() |
Images | ![]() | 4.5 MB | ||
Masks | ![]() | 40.6 MB | ![]() | |
Others | ![]() ![]() | 30.3 MB 30.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7p5cMC ![]() 7p54C ![]() 7p5jC ![]() 7p5mC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data |
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.302 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_13198_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_13198_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : TTYH3
Entire | Name: TTYH3 |
---|---|
Components |
|
-Supramolecule #1: TTYH3
Supramolecule | Name: TTYH3 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
---|---|
Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
Molecular weight | Theoretical: 116 KDa |
-Macromolecule #1: Protein tweety homolog 3
Macromolecule | Name: Protein tweety homolog 3 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 58.350234 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: SAGVSYAAPW WVSLLHRLPH FDLSWEATSS QFRPEDTDYQ QALLLLGAAA LACLALDLLF LLFYSFWLCC RRRKSEEHLD ADCCCTAWC VIIATLVCSA GIAVGFYGNG ETSDGIHRAT YSLRHANRTV AGVQDRVWDT AVGLNHTAEP SLQTLERQLA G RPEPLRAV ...String: SAGVSYAAPW WVSLLHRLPH FDLSWEATSS QFRPEDTDYQ QALLLLGAAA LACLALDLLF LLFYSFWLCC RRRKSEEHLD ADCCCTAWC VIIATLVCSA GIAVGFYGNG ETSDGIHRAT YSLRHANRTV AGVQDRVWDT AVGLNHTAEP SLQTLERQLA G RPEPLRAV QRLQGLLETL LGYTAAIPFW RNTAVSLEVL AEQVDLYDWY RWLGYLGLLL LDVIICLLVL VGLIRSSKGI LV GVCLLGV LALVISWGAL GLELAVSVGS SDFCVDPDAY VTKMVEEYSV LSGDILQYYL ACSPRAANPF QQKLSGSHKA LVE MQDVVA ELLRTVPWEQ PATKDPLLRV QEVLNGTEVN LQHLTALVDC RSLHLDYVQA LTGFCYDGVE GLIYLALFSF VTAL MFSSI VCSVPHTWQQ KRGPDEDGEE EAAPGPRQAH DSLYRVHMPS LYSCGSSYGS ETSIPAAAHT VSNAPVTEYM SQNAN FQNP RCENTPLIGR ESPPPSYTSS MRAKYLATSQ PRPDSSGSHA LEVLFQ |
-Macromolecule #3: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 3 / Number of copies: 4 / Formula: NAG |
---|---|
Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | ![]() |
---|---|
![]() | ![]() |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 Component:
| ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Vitrification | Cryogen name: ETHANE-PROPANE / Instrument: FEI VITROBOT MARK IV |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 69.56 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |